Human Cytomegalovirus DNA Quantitative Fluorescence Diagnostic Kit (PCR-Fluorescence Probing)

The diagnostic kit uses a nucleic acid lysis buffer to allow rapid lysis and release of HCMV DNA in test samples. By applying real-time fluorescence quantitative PCR technology, this test utilizes a pair of specific primers which are designed to target at a conserved sequence of HCMV-DNA, a specific fluorescence probe, accompanied with PCR mix, to achieve fast detection of HCMV-DNA through fluorescent signal changes. The PCR detection system uses UNG enzyme + dUTP contamination-proof system, which can fully degrade possible unwanted side-products, to avoid a false positive result. The PCR detection system uses internal control to judge whether the internal control is normal by monitoring the presence of PCR inhibitors, so as to avoid a false negative result.