FV Leiden / FII Real Time Duplex Mix, code AA1370/48
FV Leiden / FII Real Time Duplex Mix, code AA1370/48 Code AA1370/48 KIT Name: FV Leiden / FII Real Time Duplex Mix Description: The kit provides reagents for the genotyping of coagulation Factor V Leiden (G1691A) and Factor II (Prothrombin) (G20210A) sequence genes through the amplification of target sequences and allelic discrimination test. Uracil-DNA glycosylase (UNG) enzyme to prevent contamination. A mutation G>A in position 1691 in the Factor V gene (FV Leiden – R506Q) occurs with high frequency (20-60%) in patients with thrombosis. In fact this mutation leads to increased procoagulant activity of activated factor V, resulting in predisposition to thrombosis. Other conditions that give predisposition to the disease are environmental factors (age, pregnancy, oral contraceptive etc.). One of the most studied factors for the evaluation of thrombotic risk is Prothrombin also called Coagulation Factor II which has a main role in the coagulation cascade since prothrombin activation leads to the transformation of fibrinogen to fibrin with consequent clot formation. A mutation (G20210A) linked to high levels of activated prothrombin with consequent increased risk of venous thrombosis was identified in the 3’-UTR of the coding gene.