abia IgE total
abia IgE total enzyme immunoassay for the quantitative determination of total immunoglobulin E (IgE) concentration in human serum.
For professional use only.
IgE is the class of immunoglobulins which are found out in norm in insignificant quantities in serum and secrets (less than 0.001 % from all immunoglobulins of serum). The newborn level of the total IgE is less than 1 IU/ml. IgE levels show a slow increase during childhood, reaching adult levels in the second decade of life. In general, elevated levels of IgE indicate an increased probability of an IgE-mediated hypersensitivity, being responsible for allergic reactions. However it is necessary to mean that approximately at 30 % of patients with atopic manifestations can have normal level of the total IgE; on the contrary, raised IgE levels can be revealed at a person without an allergy.
These substances cause smooth muscle constriction and lead ultimately to allergic conditions such as wheal and flare reactions, hives, dermatitis, rhinitis, hay fever, asthma and anaphylactic shock. Infants and children with family history of atopic allergy are at increased risk of developing disease and constitute a prime population for screening.
Significant elevations may be seen in the sensitized individuals, but also in cases of myeloma, pulmonary aspergillosis, and during the active stages parasitic infections.
Principle of the test
abia IgE total is a one-step immunoassay, based on the principle of the “sandwich” method.
IgE molecules present in the sample and the labeled enzyme-anti-IgE monoclonal antibodies in the conjugate compete for binding to the capture antibody on the anti-IgE coated microplate. The enzyme activity in the antibody-bound fraction is directly proportional to the IgE concentration.
The unbound components are removed by washing. After addition of the solution containing TMB and hydrogen peroxide, the wells with bound conjugate develop a blue color which is converted to yellow after the reaction has been stopped with sulphuric acid.
The color intensity is directly proportional to the concentration of the IgE molecules in the specimen and can be read at 450 nm.