SNP Detection Real-Time PCR Kits
SNP Detection Real-Time PCR Kits The kit provides reagents in a “Ready-to-Use” master mix format which has been specifically adapted for 5’ nuclease assay. The test system is designed for use with sequence specific primers and probe. Also each master mix contains an internal control. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencer dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye which is monitored real-time by PCR detection system. The PCR cycle at which an increase in the fluorescent signal is detected initially (Ct) is proportional to the amount of the specific PCR product.