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Introduction
Cervical cancer is the fourth most common female cancer following breast, colorectal, and lung cancer. In 2018, there were about 570,000 new cases of cervical cancer and 311,000 deaths worldwide. The main cause of cervical cancer is HPV (human papillomavirus) infection. HPV is one of the most common infectious diseases that is spread by sexual contact. HPV causes genital warts, cervical intraepithelial neoplasia, and cervical carcinoma. When left untreated HPV infection, uncontrolled growth of abnormal cells can become cancer. It usually take about 10 to 15 years for persistent HPV infection to progress to cancer. Therefore, HPV test is important for early diagnosis of cervical cancer.
PaxView® HPV 20 Genotyping MPCR-ULFA Kit allows for the simultaneous detection and identification of 20 different HPV genotypes, which are 14 high risk (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68), 2 probably high risk (53, 73), and 4 low risk (6, 11, 69, 70) HPV types.
Targents
PaxView® HPV 20 Genotyping MPCR-ULFA Kit is an in vitro diagnostic kit designed for the detection and genotyping of HPV in cervical swab specimens. The kit utilizes multiplex PCR and DNA hybridization to detect and identify 20 different HPV genotypes (6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 70, 73).
Performances
DNA Extraction: 15 min
DNA Amplification: 1 hr 30min
ULFA Detection: 10 min
References
1. ICO/IARC Information Centre on HPV and Cancer. Human Papillomavirus and Related Diseases Report-World. 2019. 20
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3. J. Aveyard, M. Mehrabi, A. Cossins, H. Braven, and R. Wilson. One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow device. 2007. Chem. Commun. 4251-4253.
4. A. Chua, C. Y. Yean, M. Ravichandran B. Lim, and P. Lalitha. A rapid DNA biosensor for the molecular diagnosis of infectious disease. 2011. Biosensors and Bioelectronics. 26. 3825-3831.
5. G. Y. Lee, S. M. Kim, S. Y. Rim, H. S. Choi, C.S. Park, and J. H. Nam. Human papillomavirus (HPV) genotyping by HPV DNA chip in cervical cancer and precancerous lesions. 2005. Int. J. Gynecol Cancer. 15(1). 81– 87.