Mycobacterium Tuberculosis DNA Fluorescence Diagnostic Kit (PCR-Fluorescence Probing)
The diagnostic kit uses a nucleic acid lysis buffer to allow rapid lysis and release of TB-DNA from a sputum specimen. By applying real-time fluorescence quantitative PCR technology, this test utilizes a pair of specific primers which are designed to target at a conserved sequence of TB-DNA, a specific fluorescence probe, accompanied with PCR mix, to achieve fast detection of TB-DNA through fluorescent signal changes. The PCR detection system uses UNG enzyme + dUTP contamination-proof system, which can fully degrade possible unwanted side-products, to avoid a false positive result.