MeltPro® MTB/RIF Test Kit

MeltPro® Mycobacterium tuberculosis Rifampin-resistance Mutation Test Kit

Mycobacterium tuberculosis Rifampin-resistance Mutation Test is based on the Probe-based Melting
Curve Analysis. According to the change in T m value between targets and the corresponding wild-type
probe sequences, the test detects the mutations in rpoB gene (codons 507~533), which reports rifam-
pin-resistance information.

Detection region: mutations in rpoB codons 507~533 (81 bp)    (coverage rate: 95% of all rifampicin-resistant mutants)

Principle of Probe-Based Melting Curve Analysis

Probe-based melting curve analysis is based on melting temperature generated by thermal denaturation of
the probe-target hybrid. Melting curve plot of fluorescence (F) versus temperature (T) is transformed into
melting peak by plotting -dF/dT versus temperature. The hybridization probe with fully matched wild-type
target gives the highest T m value. With matched mutant-type target, it gives the lower T m value.
Mutation is detected as T m deviation (△T m ) compared to the wild-type hybrid.