MeltPro® MTB/FQ Test Kit
MeltPro® Mycobacterium tuberculosis Fluoroquinolones-resistance Mutation Test Kit
Mycobacterium tuberculosis Fluoroquinolones-resistance Mutation Test is suitable for rapid, qualitative
detection of fluoroquinolones-resistance mutations in Mycobacterium tuberculosis.
Mycobacterium tuberculosis Fluoroquinolones-resistance Mutation Test is based on the Probe-based
Melting Curve Analysis. According to the change in T m value between targets and the corresponding
wild-type probe sequences, the test detects the mutations in gyrA codons 88~94, which reports fluoro-
Detection region: mutations in gyrA codons 88~94 (Coverage rate: 70%-90% of all fluoroquinolone resistant mutants)
Principle of Probe-Based Melting Curve Analysis
Probe-based melting curve analysis is based on melting temperature generated by thermal denaturation of
the probe-target hybrid. Melting curve plot of fluorescence (F) versus temperature (T) is transformed into
melting peak by plotting -dF/dT versus temperature. The hybridization probe with fully matched wild-type
target gives the highest T m value. With matched mutant-type target, it gives the lower T m value.
Mutation is detected as T m deviation (△T m ) compared to the wild-type hybrid.