Hepatitis B Viral DNA Quantitative Fluorescence Diagnostic Kit (fast HBV, PCR-Fluorescence Probing) SANSURE | Advanced Molecule Diagnosis Solutions
The diagnostic kit uses a nucleic acid lysis buffer to allow rapid lysis and release of HBV-DNA from a serum or plasma specimen. By applying real-time fluorescence quantitative PCR technology, this test utilizes a pair of specific primers which are designed to target at a conserved sequence of HBV-DNA, a specific fluorescence probe, accompanied with PCR mix,to achieve quantitative detection of HBV-DNA through fluorescent signal changes. It eliminates the need to extract DNA in specimens during the entire test process. It only needs to add the serum or plasma specimen directly to PCR reaction tube and thoroughly mix it with DNA lysis buffer, which then can be used as the template for PCR amplification. This avoids laboratory contamination which occurs often during routine DNA extraction process. The PCR detection system uses UNG enzyme + dUTP contamination-proof system, which can fully degrade possible unwanted side-products, to avoid a false positive result. The PCR detection system uses an internal control, which monitors the presence of PCR inhibitors in test specimens by detecting whether the internal control signal is normal, to avoid a false negative result.