Goldstream Aspergillus Galactomannan ELISA Test

The product is an indirect competition microplate assay for the detection of Aspergillus galactomannan antigen using specific antibodies. Transferring the pre-treated mixtures of the human serum sample and the anti-galactomannan antibodies into the microplate which have been coated with the galactomannan antigen. After the microplate was incubated and washed, the enzyme labelled antibodies were added. Following a second incubation and washing, TMB substrate solution was added to form a color reaction. The absorbance value was determined by a microplate reader at 450nm wavelength. The absorbance value has a negative correlation with the content of galactomannan. Then the concentration of the galactomannan in the human serum can be calculated from the standard curve and the quantitative detection can be performed.