MeltPro® MTB/SL Test Kit
eltPro® Mycobacterium tuberculosis Second-line Injectable Drug-resistance Mutation Test Kit
Mycobacterium tuberculosis Second-line Injectable Drug-resistance Mutation Test is suitable for rapid,
qualitative detection of second-line injectable drug-resistance mutations in Mycobacterium tuberculosis.
Mycobacterium tuberculosis Second-line Injectable Drug-resistance Mutation Test is based on the
Probe-based Melting Curve Analysis. According to the change in T m value between targets and the
corresponding wild-type probe sequences, the test detects the mutations in rrs (positions 1401、1402、
1473 and 1484) and eis promoter region (positions -37、-14、-13 and -10), which reports second-line
injectable drug-resistance information.
Principle of Probe-Based Melting Curve Analysis
Probe-based melting curve analysis is based on melting temperature generated by thermal denaturation of
the probe-target hybrid. Melting curve plot of fluorescence (F) versus temperature (T) is transformed into
melting peak by plotting -dF/dT versus temperature. The hybridization probe with fully matched wild-type
target gives the highest T m value. With matched mutant-type target, it gives the lower T m value.
Mutation is detected as T m deviation (△T m ) compared to the wild-type hybrid.